The programs, configs and data will be saved onto the USB stick and preserved – due to the persistent data storage and the casper-rw file. So install any extra programs you want, configure PATHs, and download any data files you want. Now you will be inside your own BioLinux OS on the USB stick. Step 5 – Customise your BioLinux – add data and programs Exit the BIOS menu, saving any changes, and the computer should now boot into the BioLinux Live USB stick. Enter the “Boot Device Select” menu, and move your USB Stick up the boot order to the top, so that the computer will now boot from the USB stick before its own hard drive. Sometimes it is not F12, sometimes it is F10 or F2 or another key, but it should say on the screen what button to press. As soon as the first screen appears – which normally has the computer manufacturer logo – it should say something like “Press F12 to Choose Boot Device” at the bottom of the screen – so press F12 quickly before the screen disappears. Turn the computer off, insert the BioLinux Live USB stick into the computer, turn the computer back on, and get ready. Next step is to boot into the BioLinux Live USB disk from a machine – this will need to be a Windows or Linux machine, a modern Mac is unlikely to boot up from it. Next, select “USB Drive” from the “Type” drop down list, and then select your actual USB stick from the “Drive” drop down list and then click “OK” to create your bootable BioLinux Live USB stick with persistent data storage. Next, in the field entitled “Space used to preserve files across reboots (Ubuntu only)” enter “3500” into the MB textfield (3.5 GB) – you could increase this above 4GB if you have a bigger USB stick and if it is using the NTFS file system. iso file from your computer downloaded in Step 1. Select the “Diskimage” toggle button, select “ISO” from the drop down list, and then navigate to and select the BioLinux. Insert your blank USB key into your computer. Launch unetbootin. To play safe, the USB stick should probably be in FAT32 format – FAT32 has a limitation of 4GB for file sizes – this includes the overall casper-rw BioLinux file which will be where all the persistent data is stored, so if you are going to be storing more than 4GB of data then you will probably need the NTFS file system on the USB stick. iso file is 3.58GB in size, a USB stick of atleast 4GB is needed, but that is a little to close for comfort, so best to go for a USB stick of atleast 8GB these days 8GB sticks are very cheap (£2.99) and are the same price (if not cheaper) as 4GB sticks. Step 3 – Create an initial BioLinux Live USB disk with persistent dataĪs the. It is simple to install, on a Mac you just move the downloaded unetbootin.app file into /Applications UNetbootin allows you to create bootable Live USB drives for Ubuntu and other Linux distributions without burning a CD. The bio-linux-8-latest.iso image is currently (March 2016) 3.58GB in size. iso file is an archive file that contains the whole BioLinux operating system – it can be used later to either install BioLinux onto a machine, or to create a bootable BioLinux USB Live disk. Step 1 – Download the BioLinux ISO file for use with DVD/USB media This was used for a course so that each stick had the same NGS data and the same additional (non-BioLinux) programs pre-installed and already configured. These are the steps I used to create a batch of bootable BioLinux Live USB sticks – with persistent data so that any data files created/downloaded would be preserved. How to make a BioLinux Live USB Stick – with persistent data storage
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